1MQG

Crystal Structure of the GluR2 Ligand Binding Core (S1S2J) in Complex with Iodo-Willardiine at 2.15 Angstroms Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2001-04-11
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	48.006, 88.716, 58.907
Unit cell angles	90.00, 99.24, 90.00

Refinement procedure

Resolution	30.000 * - 2.150
R-factor	0.185
Rwork	0.185
R-free	0.23700 *
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1mqi
RMSD bond length	0.006
RMSD bond angle	1.240 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.250
High resolution limit [Å]	2.150	2.150
Rmerge	0.064	0.157
Number of reflections	23506
Completeness [%]	88.5	50.5
Redundancy	3.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	4 *	PEG 1000, Phosphate-citrate buffer, Li sulfate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mM)
2	1	reservoir	PEG1000	18-22 (%)
3	1	reservoir	lithium sulfate	0.2 (M)
4	1	reservoir	phosphate-citrate	0.1 (M)	pH4.2