1MQ0
Crystal Structure of Human Cytidine Deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 52.398, 55.680, 90.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.450 - 2.400 |
| Rwork | 0.217 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jtk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.700 * |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.330 * |
| Number of reflections | 22165 * | |
| <I/σ(I)> | 10.4 | 2.9 |
| Completeness [%] | 97.1 | 98.1 |
| Redundancy | 4.4 * | 4.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | sodium acetate, 2-methyl-2,4-pentane-diol, calcium chloride, synthetic inhibitor, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 36.3 (mg/ml) | |
| 2 | 1 | drop | inhibitor | 6.25 (mM) | |
| 3 | 1 | drop | beta-mercaptoethanol | 5 (mM) | pH7.5 |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | drop | Tris | 20 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 7 | 1 | reservoir | MPD | 28-32 (%) | |
| 8 | 1 | reservoir | 10 (mM) |
