1MPU
Crystal Structure of the free human NKG2D immunoreceptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-12-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.650, 87.650, 36.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.245 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HYR (CHAIN A) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 * | 0.199 * |
| Number of reflections | 5057 | 466 * |
| <I/σ(I)> | 20.2 | 6.8 |
| Completeness [%] | 96.5 | 90.1 |
| Redundancy | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | 291 | ammonium dihydrogen phosphate, bicine, ethylene glycol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | huNKG2D | 5 (mg/ml) | |
| 2 | 1 | drop | ULBP1 | 4 (mg/ml) | |
| 3 | 1 | drop | PIPES | 25 (mM) | pH7.0 |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | EDTA | 1 (mM) | |
| 6 | 1 | drop | 0.02 (%) | ||
| 7 | 1 | reservoir | ammonium dihydrogen phosphate/diammonium hydrogen phosphate | 1.6 (M) | |
| 8 | 1 | reservoir | ethylene glycol | 10 (%) | |
| 9 | 1 | reservoir | Bicine | 100 (mM) | pH9.0 |
