1MPQ

MALTOPORIN TREHALOSE COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-20
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-04-12
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	130.000, 212.200, 218.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.000
R-factor	0.218
Rwork	0.218
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mal
RMSD bond length	0.020
RMSD bond angle	28.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.078	0.280
Number of reflections	59057
<I/σ(I)>		2.5
Completeness [%]	97.7	93.2
Redundancy	3.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		pH 7.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	beta-D-decylmaltoside	0.4 (%(w/w))
2	1	1	HEPES	20 (mM)
3	1	1		0.1 (M)
4	1	1	PEG2000	11.0-12.5 (%(w/w))