1MPN

MALTOPORIN MALTOTRIOSE COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-10-18
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	131.900, 214.800, 220.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.200
R-factor	0.218
Rwork	0.218
R-free	0.23100
RMSD bond length	0.016
RMSD bond angle	28.400 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000
High resolution limit [Å]	3.200	3.200 *
Rmerge	0.095	0.351 *
Number of reflections	51209
Completeness [%]	95.2	99.8 *
Redundancy	3.7	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	beta-D-decylmaltoside	0.4 (%(w/w))
2	1	1	HEPES	20 (mM)
3	1	1		0.1 (M)
4	1	1	PEG2000	11.0-12.5 (%(w/w))