1MOY

Streptavidin Mutant with Osteopontin Hexapeptide Insertion Including RGD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-10
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	I 41 2 2
Unit cell lengths	56.900, 56.900, 170.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.550
R-factor	0.132
Rwork	0.132
R-free	0.18900 *
Structure solution method	isomorphous
RMSD bond length	0.010
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.076	0.593
Total number of observations	486781 *
Number of reflections	20871 *
Completeness [%]	99.9	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MPD	40-50 (%)