1MOW
E-DreI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Detector technology | CCD |
| Collection date | 2002-02-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 131.760, 131.760, 120.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.860 - 2.400 |
| Rwork | 0.231 |
| R-free | 0.25600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | E-DreI computational model; I-CreI/DNA complex (RCSB 1G9Z) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.070 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.081 |
| Total number of observations | 305673 * |
| Number of reflections | 91688 * |
| Completeness [%] | 99.0 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | ammonium sulphate, MES, magnesium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium sulphate | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 2 | MgCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.1-2.4 (M) | |
| 2 | 1 | reservoir | 100 (mM) | pH6.5 | |
| 3 | 1 | reservoir | 10 (mM) |
