1MOH

FERRIC MONOMERIC HEMOGLOBIN I (HB I)

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-12-14
Detector	RIGAKU
Spacegroup name	P 1 21 1
Unit cell lengths	50.550, 38.790, 42.510
Unit cell angles	90.00, 106.93, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.186 *
RMSD bond length	0.015
RMSD bond angle	1.760
Data reduction software	MOSFLM
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.080
Total number of observations	28787 *
Number of reflections	12135
Completeness [%]	96.7 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *	Casale, E., (1991) J. Mol. Biol., 222, 447. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	succinate	0.05 (M)
3	1	reservoir	ammonium sulfate	3.3 (M)