1MNN
Structure of the sporulation specific transcription factor Ndt80 bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2001-06-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98008 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.143, 78.887, 161.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 * - 1.400 |
| R-factor | 0.19544 |
| Rwork | 0.195 |
| R-free | 0.20600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.520 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.051 * | 0.493 * |
| Total number of observations | 3338673 * | |
| Number of reflections | 88384 | |
| <I/σ(I)> | 35 | 3 |
| Completeness [%] | 99.8 | 97.8 |
| Redundancy | 38. * | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 400, calcium chloride, sodium chloride, bis-tris propane, DTT, spermine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | CaCl2 | ||
| 3 | 1 | 1 | NaCl | ||
| 4 | 1 | 1 | bis-tris propane | ||
| 5 | 1 | 1 | DTT | ||
| 6 | 1 | 1 | spermine | ||
| 7 | 1 | 2 | CaCl2 | ||
| 8 | 1 | 2 | NaCl |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 30 (%) | |
| 3 | 1 | reservoir | Bis-Tris-propane | 50 (mM) | pH7.0 |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | 1.5 (mM) | ||
| 7 | 1 | reservoir | dithiothreitol | 1 (mM) |
