1MNM

YEAST MATALPHA2/MCM1/DNA TERNARY TRANSCRIPTION COMPLEX CRYSTAL STRUCTURE

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1996-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	70.620, 72.550, 150.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.250
R-factor	0.24
Rwork	0.240
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1SRS AND PDB ENTRY 1APL
RMSD bond length	0.006
RMSD bond angle	1.200
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.065	0.364
Total number of observations	327727 *
Number of reflections	35613
<I/σ(I)>	9.8	1.7
Completeness [%]	94.6	0.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5		pH 5.50