1MNC
STRUCTURE OF HUMAN NEUTROPHIL COLLAGENASE REVEALS LARGE S1' SPECIFICITY POCKET
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.920, 61.280, 68.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.062 |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Rmerge | 0.053 * |
| Total number of observations | 26807 * |
| Number of reflections | 8593 * |
| Completeness [%] | 95.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 * | Spurlino, J.C., (1994) Proteins Struct. Funct. Genet., 19, 98. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 2.5 (mM) | |
| 3 | 1 | 1 | 5.0 (mM) | ||
| 4 | 1 | 1 | 0.4 (M) | ||
| 5 | 1 | 1 | 3 (mM) | ||
| 6 | 1 | 1 | hydroxamate inhibitor | 1 (mM) |
