1MN9

NDP kinase mutant (H122G) complex with RTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-06-10
Detector	MARRESEARCH
Wavelength(s)	1.542
Spacegroup name	P 31 2 1
Unit cell lengths	71.731, 71.731, 153.818
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.200 - 2.900
Rwork	0.207
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.117 *
Total number of observations	93018 *
Number of reflections	10566 *
Completeness [%]	99.1	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	291	PEG 550, Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG550 MME	13 (%)
2	1	drop	Tris-HCl	100 (mM)	pH7.5
3	1	drop		30 (mM)
4	1	drop	protein	10 (mg/ml)
5	1	drop	RTP	20 (mM)
6	1	reservoir	PEG550 MME	26 (%)