1MMX

Crystal structure of galactose mutarotase from Lactococcus lactis complexed with D-fucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	AREA DETECTOR
Collection date	2002-02-16
Detector	SIEMENS HI-STAR
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.900, 76.300, 210.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.171
Rwork	0.168
R-free	0.22900
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1l7j
RMSD bond length	0.012
RMSD bond angle	18.600 *
Data reduction software	SAINT
Data scaling software	SAINT
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.047 *	0.246 *
Number of reflections	66843	7954 *
<I/σ(I)>	15.3	3.1
Completeness [%]	97.5	94.4
Redundancy	2.6	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	277	PEG-5000-OMe, MES, fucose, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG5000 MME	15-19 (%)
2	1	reservoir	MES	100 (mM)	pH6.0
3	1	drop	enzyme	17.5 (mg/ml)