1MMQ

MATRILYSIN COMPLEXED WITH HYDROXAMATE INHIBITOR

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1993-10-05
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	61.900, 61.900, 88.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.179
Rwork	0.179
RMSD bond length	0.013
RMSD bond angle	2.730
Data reduction software	MOSFLM (V. 5.1.2)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.120
Total number of observations	83992 *
Number of reflections	15546
Completeness [%]	92.7
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	17-25 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	inhibitor	1.2 (mM)
3	1	drop	DMSO	2.5 (%)
4	1	reservoir		0.2 (M)
5	1	reservoir	HEPES	0.1 (M)
6	1	reservoir	2-propanol	30 (%)