1MMN
X-RAY STRUCTURES OF THE MGADP, MGATPGAMMAS, AND MGAMPPNP COMPLEXES OF THE DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 113 |
| Collection date | 1996-05 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.900, 180.100, 54.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mmd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.600 |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.075 | |
| Total number of observations | 244680 * | |
| Number of reflections | 58467 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 92.6 | 55.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 8.3 (%) | |
| 2 | 1 | 1 | HEPES | 50 (mM) | |
| 3 | 1 | 1 | 125 (mM) | ||
| 4 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 5 | 1 | 1 | 1.2 (mM) | ||
| 6 | 1 | 1 | nucleotide | 1.2 (mM) | |
| 7 | 1 | 1 | protein | 5 (mg/ml) |
