1MML

MECHANISTIC IMPLICATIONS FROM THE STRUCTURE OF A CATALYTIC FRAGMENT OF MMLV REVERSE TRANSCRIPTASE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Detector technology	IMAGE PLATE
Collection date	1993-08-28
Detector	FUJI
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	84.100, 84.100, 74.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.197 *
R-free	0.25900 *
RMSD bond length	0.014 *
RMSD bond angle	0.032 *
Data reduction software	DENZO
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.038
Total number of observations	63951 *
Number of reflections	27086 *
Completeness [%]	96.3 *
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *	used as seeds *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	MES	50 (mM)
3	1	drop		0.25 (M)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	Tris	50 (mM)
6	1	reservoir		0.2 (M)
7	1	reservoir	PEG4000	20 (%)