1MMD
TRUNCATED HEAD OF MYOSIN FROM DICTYOSTELIUM DISCOIDEUM COMPLEXED WITH MGADP-BEF3
Experimental procedure
| Source type | ROTATING ANODE |
| Collection date | 1994-05 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 105.300, 182.600, 54.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.260 |
| Data reduction software | DENZO |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 * |
| Rmerge | 0.033 |
| Number of reflections | 63717 * |
| Completeness [%] | 88.0 * |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4-5 (mg/ml) | |
| 10 | 1 | 1 | DTT | 3 (mM) | |
| 2 | 1 | 1 | PEG8000 | 8.5 (%) | |
| 3 | 1 | 1 | HEPES | 25 (mM) | |
| 4 | 1 | 1 | methylpentanediol | 1.5 (%) | |
| 5 | 1 | 1 | 170 (mM) | ||
| 6 | 1 | 1 | ADP | 1.8 (mM) | |
| 7 | 1 | 1 | 1.8 (mM) | ||
| 8 | 1 | 1 | 1.8 (mM) | ||
| 9 | 1 | 1 | 9 (mM) |
