1MMB

COMPLEX OF BB94 WITH THE CATALYTIC DOMAIN OF MATRIX METALLOPROTEINASE-8

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	33.670, 69.640, 73.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.189
Rwork	0.189
RMSD bond length	0.015
RMSD bond angle	1.900
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.100 *	2.100 *
Rmerge	0.103
Total number of observations	33465 *
Number of reflections	9465
Completeness [%]	89.1	34.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HNC	8 (mg/ml)
2	1	drop	Mes/NaOH	3 (mM)
3	1	drop		100 (mM)
4	1	drop		5 (mM)
5	1	drop		0.02 (%)
6	1	drop	batimaster/PEG6000		10 % m/v PEG6000 in 0.2 M Mes/NaOH at pH 6.0 with ca. 0.5 mg/L batimastat
7	1	reservoir	potassium phosphate	0.8 (M)
8	1	reservoir		0.02 (%)