1MMA
X-RAY STRUCTURES OF THE MGADP, MGATPGAMMAS, AND MGAMPPNP COMPLEXES OF THE DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 113 |
| Collection date | 1995-08 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 103.600, 179.000, 53.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.219 * |
| Rwork | 0.219 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mmd |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.500 |
| Phasing software | AMoRE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.046 | |
| Total number of observations | 189699 * | |
| Number of reflections | 56984 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 96.2 | 90.5 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 8.3 (%) | |
| 2 | 1 | 1 | HEPES | 50 (mM) | |
| 3 | 1 | 1 | 125 (mM) | ||
| 4 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 5 | 1 | 1 | 1.2 (mM) | ||
| 6 | 1 | 1 | nucleotide | 1.2 (mM) | |
| 7 | 1 | 1 | protein | 5 (mg/ml) |
