1MM9

Streptavidin Mutant with Insertion of Fibronectin Hexapeptide, including RGD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-01-29
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	I 41 2 2
Unit cell lengths	56.900, 56.900, 170.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.650 *
R-factor	0.145 *
Rwork	0.134
R-free	0.19500 *
Structure solution method	isomorphous
RMSD bond length	0.009
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.650 *	1.650 *
Rmerge	0.068	0.495
Total number of observations	240518 *
Number of reflections	17197
<I/σ(I)>	28.4	3.2
Completeness [%]	98.9 *	81.4
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	MPD	40-50 (%)