1MM6

crystal structure of the GluR2 ligand binding core (S1S2J) in complex with quisqualate in a non zinc crystal form at 2.15 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-04-19
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	98.560, 121.530, 47.451
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.150
R-factor	0.19
Rwork	0.190
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1mqi
RMSD bond length	0.005
RMSD bond angle	1.163 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.150	2.150
Rmerge	0.055	0.129
Number of reflections	28647
Completeness [%]	90.3	59.9 *
Redundancy	3.39

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5	4 *	PEG 8K, ammonium sulfate, sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	quisqualate	5 (mM)
3	1	reservoir	PEG8000	10-15 (%)
4	1	reservoir	zinc acetate	0.1 (M)
5	1	reservoir	sodium acetate	0.1 (M)	pH5.5