1MLU

NITRIC OXIDE RECOMBINATION TO DOUBLE MUTANTS OF MYOGLOBIN: THE ROLE OF LIGAND DIFFUSION IN A FLUCTUATING HEME POCKET

Experimental procedure

Spacegroup name	P 6
Unit cell lengths	91.200, 91.200, 45.870
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.143
Rwork	0.143
RMSD bond length	0.024
RMSD bond angle	2.654
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.037 *
Completeness [%]	87.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	ammonium sulfate	2.6-2.8 (M)
3	1	reservoir	Tris-HCl	20 (mM)
4	1	reservoir	EDTA	1 (mM)