1ML8
structural genomics
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-21 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97945; 0.97934 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 76.352, 76.352, 107.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.070 - 2.600 |
| R-factor | 0.22976 |
| Rwork | 0.228 |
| R-free | 0.26790 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.693 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.05) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.530 |
| Number of reflections | 10742 | |
| <I/σ(I)> | 27 | 2.8 |
| Completeness [%] | 99.0 | 95.8 |
| Redundancy | 5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% PEG4K;0.2M Ammon. Sulph., pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
