1MKZ

Crystal structure of MoaB protein at 1.6 A resolution.

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Detector	CUSTOM-MADE
Spacegroup name	P 3 2 1
Unit cell lengths	69.169, 69.169, 126.184
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	129.100 - 1.600
R-factor	0.18461
Rwork	0.183
R-free	0.21900 *
RMSD bond length	0.020
RMSD bond angle	1.885
Data reduction software	HKL-2000
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.100
High resolution limit [Å]	1.600
Rmerge	0.057 *	0.450 *
Total number of observations	311387 *
Number of reflections	55534 *
Completeness [%]	99.4 *	99 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.0 (M)
2	1	reservoir	ethylene glycol	20 (%(v/v))