1MKT
CARBOXYLIC ESTER HYDROLASE, 1.72 ANGSTROM TRIGONAL FORM OF THE BOVINE RECOMBINANT PLA2 ENZYME
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS II |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-24 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 46.780, 46.780, 102.890 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.720 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.28400 |
| Starting model (for MR) | RECOMBINANT PLA2 (PDB ENTRY 2BPP) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.700 * |
| Data reduction software | R-AXIS (IIC) |
| Data scaling software | R-AXIS (II) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.800 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.079 | 0.212 |
| Total number of observations | 37578 * | |
| Number of reflections | 11516 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 79.4 | 55 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | CRYSTALS WERE GROWN BY THE VAPOR DIFFUSION METHOD USING THE CONDITIONS 5 (MICRO)L OF THE MUTANT PROTEIN (15MG/ML OF THE PROTEIN), 5MM CACL2, 50MM TRIS BUFFER, PH 7.2 AND 2 (MICRO)L OF 75% MPD AND (50%) OF MPD IN THE RESERVOIR, vapor diffusion |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 11 (mg/ml) | |
| 2 | 1 | drop | 4 (mM) | ||
| 3 | 1 | drop | Tris | 36 (mM) | |
| 4 | 1 | drop | MPD | 21 (%) | |
| 5 | 1 | reservoir | MPD | 50 (%) |
