1MKJ

Human Kinesin Motor Domain With Docked Neck Linker

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-10-04
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	73.796, 74.086, 91.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.030 - 2.700
R-factor	0.209
Rwork	0.209
R-free	0.25900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kin
RMSD bond length	0.007
RMSD bond angle	23.200 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.079 *	0.270 *
Total number of observations	247497 *
Number of reflections	14266 *
<I/σ(I)>	23	6.9
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	277	1.5-2 M Li2SO4, 25 mM HEPES pH 7.5, 0-50 mM KCl, pH 7.50, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	HEPES	25 (mM)	pH6.8
2	1	drop		150 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	drop		2 (mM)
5	1	drop	protein	10 (mg/ml)
6	1	reservoir		15.2-2 (M)
7	1	reservoir	HEPES	25 (mM)	pH7.5
8	1	reservoir		0-50 (mM)