1MK2
SMAD3 SBD complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2000-01-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.995, 71.716, 86.886 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.440 - 2.800 * |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26700 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.510 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.440 | 2.800 |
High resolution limit [Å] | 2.800 * | 2.800 * |
Rmerge | 0.139 * | 0.464 * |
Total number of observations | 48600 * | |
Number of reflections | 7498 * | 556 * |
<I/σ(I)> | 3.12 | |
Completeness [%] | 0.9 | 99.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.4 * | ||
1 | Vapor diffusion, hanging drop * | 7.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 1.8 (M) | ||
2 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.0 |
3 | 1 | drop | Tris | 20 (mM) | pH7.4 |
4 | 1 | drop | 10 (mM) | ||
5 | 1 | drop | EDTA | 0.1 (mM) | |
6 | 1 | drop | dithiothreitol | 1 (mM) |