1MJX

STRUCTURE OF INORGANIC PYROPHOSPHATASE MUTANT D65N

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1996-04
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	110.200, 110.200, 155.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.150
Rwork	0.190
R-free	0.26300
RMSD bond length	0.014
RMSD bond angle	0.037
Refinement software	REFMAC

Data quality characteristics

	Overall
High resolution limit [Å]	2.150 *
Rmerge	0.116
Total number of observations	264155 *
Number of reflections	19966
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		Avaeva, S., (1997) FEBS Lett., 410, 502. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	0.1 (M)
2	1	drop	ammonium sulfate	35 (%)
3	1	drop	protein	4 (mg/ml)
4	1	reservoir	Tris-HCl	0.1 (M)
5	1	reservoir	ammonium sulfate	45 (%)