1MJP
METHIONINE APOREPRESSOR MUTANT (Q44K) COMPLEXED TO THE MINIMAL MET CONSENSUS OPERATOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 118.390, 118.390, 83.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.30900 |
| Structure solution method | DIFFERENCE FOURIER METHODS |
| Starting model (for MR) | 1cma |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.860) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.085 | 0.085 |
| Number of reflections | 19851 | |
| <I/σ(I)> | 11.5 | 3.3 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | PROTEIN (10MG/ML) + DNA (4MG/ML) WAS CRYSTALLISED FROM 40% MPD, 100MM SODIUM CACODYLATE BUFFER, PH 7.0. |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PROTEIN | ||
| 2 | 1 | 1 | DNA | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 1 | SODIUM CACODYLATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 4 (mg/ml) | ||
| 3 | 1 | drop | SAM | 1.5 (mg/ml) | |
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | sodium cacodylate | 10 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 20 (mM) | |
| 8 | 1 | reservoir | MPD | 28-38 (%) |
