1MJC

CRYSTAL STRUCTURE OF CSPA, THE MAJOR COLD SHOCK PROTEIN OF ESCHERICHIA COLI

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	47.120, 39.920, 30.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.187
Rwork	0.187
RMSD bond length	0.014
RMSD bond angle	0.047 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.063 *	0.113 *
Total number of observations	15912 *
Number of reflections	3785 *
Completeness [%]	88.9 *	87.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		50 (mM)
4	1	reservoir	PEG1500	26 (%)