1MJ0
SANK E3_5: an artificial Ankyrin repeat protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 73.864, 47.360, 47.003 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.030 * |
| R-factor | 0.18432 |
| Rwork | 0.179 |
| R-free | 0.23000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1awc |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.926 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.090 |
| High resolution limit [Å] | 2.030 * | 2.020 |
| Rmerge | 0.100 * | 0.330 |
| Total number of observations | 173985 * | |
| Number of reflections | 11291 * | |
| <I/σ(I)> | 20.3 | 48.8 |
| Completeness [%] | 98.4 * | 83.8 |
| Redundancy | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG 4000, Li2SO4, NH4OAc, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | pH8.0 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | protein | 9 (mg/ml) | |
| 4 | 1 | reservoir | PEG4000 | 16-22 (%) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | 0.1 (M) | pH4.6-5.0 |
