1MIY

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-15
Detector	MARRESEARCH
Wavelength(s)	0.9789
Spacegroup name	P 31 2 1
Unit cell lengths	105.552, 105.552, 182.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 * - 3.500 *
R-factor	0.29104
Rwork	0.286
R-free	0.33100 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.460
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.17)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.500
Rmerge	0.048
Total number of observations	155697 *
Number of reflections	15352
<I/σ(I)>	32.6
Completeness [%]	99.8 *	99.9 *
Redundancy	10.14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7.5	277	NaCl, Tris, MgCl2, pH 7.5, MICRODIALYSIS at 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	8 (mg/ml)
2	1	2	Tris	50 (mM)	pH7.5
3	1	2		200 (mM)
4	1	2		10 (mM)