1MIM

IGG FAB FRAGMENT (CD25-BINDING)

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	39.580, 59.760, 102.090
Unit cell angles	90.00, 99.98, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.196
Rwork	0.196
RMSD bond length	0.014
RMSD bond angle	3.380
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.600
Rmerge	0.057 *
Total number of observations	32477 *
Number of reflections	14331
Completeness [%]	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop	PEG8000	7.5 (%(w/v))
3	1	drop		0.02 (%(w/v))
4	1	drop	protein	0.33 (mM)
5	1	reservoir	Tris-HCl	100 (mM)
6	1	reservoir	PEG8000	15.0 (%(w/v))
7	1	reservoir		0.04 (%(w/v))