1MIL
TRANSFORMING PROTEIN
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 26.370, 41.550, 97.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.870 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.051 * |
| Total number of observations | 7344 * |
| Number of reflections | 3028 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | 22 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 50 (mM) | ||
| 2 | 1 | drop | PEG8000 | 9 (%(w/v)) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 0.02 (%(w/v)) | ||
| 5 | 1 | drop | Shc | 1 (mM) | |
| 6 | 1 | reservoir | 100 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 18 (%(w/v)) | |
| 8 | 1 | reservoir | 0.04 (%(w/v)) |
