1MI8

2.0 Angstrom crystal structure of a DnaB intein from Synechocystis sp. PCC 6803

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-05-08
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	58.170, 58.170, 70.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 * - 2.000
R-factor	0.226
Rwork	0.210
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	2.040
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000 *	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.046 *	0.262 *
Total number of observations	107903 *
Number of reflections	9307
Completeness [%]	97.6 *	75.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	PEG4000, Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	100 (mM)	pH7.5-8.0
2	1	reservoir	PEG4000	16-20 (%)
3	1	reservoir	ethylene glycol	4 (%(v/v))