1MI4
Glyphosate insensitive G96A mutant EPSP synthase liganded with shikimate-3-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.760, 85.090, 87.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.700 |
| R-factor | 0.157 |
| Rwork | 0.157 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g6t |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.720 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.054 | 0.346 |
| Total number of observations | 199475 * | |
| Number of reflections | 47038 | 3815 * |
| Completeness [%] | 97.5 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 19 * | sodium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 50 (mM) | pH7.8 |
| 2 | 1 | drop | dithiothreitol | 2 (mM) | |
| 3 | 1 | drop | protein | 100 (mg/ml) | |
| 4 | 1 | reservoir | sodium formate | 2 (M) | pH7. |
| 5 | 1 | reservoir | S3P | 5 (mM) | |
| 6 | 1 | reservoir | glyphosate | 20 (mM) |
