1MHY

METHANE MONOOXYGENASE HYDROXYLASE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1994-05-10
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	264.490, 71.190, 139.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.000
R-factor	0.137
Rwork	0.137
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CHAINS B D AND G OF PDB ENTRY 1MMO
RMSD bond length	0.016
RMSD bond angle	21.140 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Rmerge	0.075
Total number of observations	391667 *
Number of reflections	87489
<I/σ(I)>	12.5
Completeness [%]	96.8
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			used to seeding, Froland, W.A., (1994) J. Mol. Biol., 236, 379. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MMOH	2.5-3.5 (mg/ml)
2	1	drop	sodium phosphate/citrate	50 (mM)
3	1	drop	PEG2000	9-11 (%)
4	1	reservoir	PEG2000	18-22 (%)