1MHT
COVALENT TERNARY STRUCTURE OF HHAI METHYLTRANSFERASE, DNA AND S-ADENOSYL-L-HOMOCYSTEINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS |
| Synchrotron site | NSLS |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 99.860, 99.860, 325.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 * |
| R-factor | 0.174 |
| Rwork | 0.174 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 * |
| Data reduction software | MADNES |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.076 |
| Number of reflections | 17839 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.25 | 16 * | BIS-TRIS-PROPANE_HCL, NACL, EDTA, pH 7.25, VAPOR DIFFUSION, HANGING DROP, temperature 289.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE_HCL | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | EDTA | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | NH4 SULFATE | ||
| 7 | 1 | 2 | NA CITRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | Na2EDTA | 0.5 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.2-1.4 (M) | |
| 6 | 1 | reservoir | sodium citrate | 50 (mM) |
