1MHE
THE HUMAN NON-CLASSICAL MAJOR HISTOCOMPATIBILITY COMPLEX MOLECULE HLA-E
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 182.200, 182.200, 88.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.850 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hla |
| RMSD bond length | 0.011 |
| RMSD bond angle | 26.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.100 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.101 | 0.483 |
| Total number of observations | 248512 * | |
| Number of reflections | 37611 | |
| <I/σ(I)> | 14.25 | 2.1 |
| Completeness [%] | 95.6 | 85.7 |
| Redundancy | 6.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | 18 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 10 (mM) | |
| 4 | 1 | reservoir | PEG400 | 2 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) |
