1MHD
CRYSTAL STRUCTURE OF A SMAD MH1 DOMAIN BOUND TO DNA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-05 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.600, 60.400, 71.600 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.28800 |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.734 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.053 * | 0.162 * |
| Total number of observations | 39338 * | |
| Number of reflections | 9228 | |
| <I/σ(I)> | 33 | 9 |
| Completeness [%] | 97.0 | 96.5 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | citrate | 50 (mM) | |
| 2 | 1 | reservoir | PEG2000 | 28 (%) | |
| 3 | 1 | reservoir | ammonium acetate | 100 (mM) | |
| 4 | 1 | reservoir | 5 (mM) | ||
| 5 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 6 | 1 | drop | protein | 1 (mM) |
