1MHC
MODEL OF MHC CLASS I H2-M3 WITH NONAPEPTIDE FROM RAT ND1 REFINED AT 2.3 ANGSTROMS RESOLUTION
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.250, 66.100, 55.170 |
| Unit cell angles | 102.71, 96.28, 110.19 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.880 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.100 | 2.100 * |
| Rmerge | 0.079 | |
| Number of reflections | 33706 | |
| Completeness [%] | 70.0 | 43 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 26 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 16-18 (%) | |
| 4 | 1 | reservoir | HEPES | 100 (mM) | |
| 5 | 1 | reservoir | MPD | 2 (%) |
