1MH9

Crystal Structure Analysis of deoxyribonucleotidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	CCD
Collection date	2001-02-03
Detector	MARRESEARCH
Wavelength(s)	1.03
Spacegroup name	P 43 21 2
Unit cell lengths	73.965, 73.965, 105.932
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.640 - 1.800
R-factor	0.159
Rwork	0.159
R-free	0.19000 *
Structure solution method	MAD
RMSD bond length	0.020 *
RMSD bond angle	2.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.710
High resolution limit [Å]	1.650	1.650
Rmerge	0.077 *	0.346 *
Number of reflections	27898
Completeness [%]	100.0 *	100
Redundancy	7.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	PEG 8000, potassium phosphate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris	20 (mM)	pH7.5
2	1	drop	glycerol	20 (%(v/v))
3	1	drop	dithiothreitol	2 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop		75 (mM)
6	1	drop	protein	13 (mg/ml)
7	1	reservoir	PEG8000	15-25 (%(w/v))
8	1	reservoir		0.05 (M)	pH5.3