1MH9
Crystal Structure Analysis of deoxyribonucleotidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 73.965, 73.965, 105.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.640 - 1.800 |
| R-factor | 0.159 |
| Rwork | 0.159 |
| R-free | 0.19000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.020 * |
| RMSD bond angle | 2.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.077 * | 0.346 * |
| Number of reflections | 27898 | |
| Completeness [%] | 100.0 * | 100 |
| Redundancy | 7.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | PEG 8000, potassium phosphate, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 20 (mM) | pH7.5 |
| 2 | 1 | drop | glycerol | 20 (%(v/v)) | |
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | 75 (mM) | ||
| 6 | 1 | drop | protein | 13 (mg/ml) | |
| 7 | 1 | reservoir | PEG8000 | 15-25 (%(w/v)) | |
| 8 | 1 | reservoir | 0.05 (M) | pH5.3 |
