1MH3

maltose binding-a1 homeodomain protein chimera, crystal form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 43 21 2
Unit cell lengths	95.485, 95.485, 155.887
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 * - 2.100
Rwork	0.232
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MBP (pdb code 4MBP)
RMSD bond length	0.006
RMSD bond angle	1.160 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000 *	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.057 *	0.216 *
Number of reflections	42612
<I/σ(I)>	30.1	4.9
Completeness [%]	99.2	97.1
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	amonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	MES	100 (mM)	pH5.0
3	1	reservoir	ammonium sulfate	2.4 (M)