1MGQ
CRYSTAL STRUCTURE OF A HEPTAMERIC SM-LIKE PROTEIN FROM METHANOBACTERIUM THERMOAUTOTROPHICUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.591, 71.883, 95.095 |
| Unit cell angles | 90.00, 94.02, 90.00 |
Refinement procedure
| Resolution | 14.890 - 1.700 |
| R-factor | 0.2309 |
| Rwork | 0.229 |
| R-free | 0.26120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i81 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.860 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.490 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 59871 | |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | PEG 3350, TRIS, LITHIUM SULPHATE, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
