1MG9
The structural basis of ClpS-mediated switch in ClpA substrate recognition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 93.654, 93.654, 78.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.300 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lzw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.700 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 2.300 | |
| Rmerge | 0.082 * | 0.272 * |
| Total number of observations | 75277 * | |
| Number of reflections | 28042 * | |
| Completeness [%] | 94.5 * | 96.8 * |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 278 * | Zeth, K., (2002) Acta Crystallogr., D58, 1207. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 20 (mM) | ||
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | 2-propanol | 20 (%) | |
| 6 | 1 | reservoir | PEG4000 | 20 (%) | |
| 7 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 8 | 1 | drop | spermine | 10 (mM) |
