1MG9

The structural basis of ClpS-mediated switch in ClpA substrate recognition

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-06
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 41 21 2
Unit cell lengths	93.654, 93.654, 78.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.252
Rwork	0.252
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lzw
RMSD bond length	0.007
RMSD bond angle	19.700 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.300
Rmerge	0.082 *	0.272 *
Total number of observations	75277 *
Number of reflections	28042 *
Completeness [%]	94.5 *	96.8 *
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	278 *	Zeth, K., (2002) Acta Crystallogr., D58, 1207. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop		20 (mM)
2	1	drop		20 (mM)
3	1	drop	HEPES	10 (mM)	pH7.5
4	1	drop	protein	10 (mg/ml)
5	1	reservoir	2-propanol	20 (%)
6	1	reservoir	PEG4000	20 (%)
7	1	reservoir	sodium citrate	0.1 (M)	pH5.6
8	1	drop	spermine	10 (mM)