1MG6
The Crystal Structure of a K49 PLA2 from the Snake Venom of Agkistrodon acutus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-17 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 45.300, 59.550, 46.130 |
| Unit cell angles | 90.00, 117.69, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.600 |
| R-factor | 0.221 |
| Rwork | 0.197 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1clp |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.051 | 0.226 |
| Number of reflections | 13205 | |
| Completeness [%] | 91.8 | 77.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 20%PEG4000, 20%Isopropanol, 0.1M tri-sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
