1MG3
MUTATION OF ALPHA PHE55 OF METHYLAMINE DEHYDROGENASE ALTERS THE REORGANIZATION ENERGY AND ELECTRONIC COUPLING FOR ITS ELECTRON TRANSFER REACTION WITH AMICYANIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-12 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.520, 188.372, 127.366 |
| Unit cell angles | 90.00, 98.84, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.193 |
| R-free | 0.24600 |
| Structure solution method | refined directly |
| Starting model (for MR) | mutant complex structure |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.420 * |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.093 | 0.246 |
| Number of reflections | 117875 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 81.3 | 50.7 |
| Redundancy | 3.7 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | alphaF55A MADH | 9.6 (mg/ml) | |
| 2 | 1 | drop | amicyanin | 2.9 (mg/ml) | |
| 3 | 1 | drop | cytochrome c-551i | 4.8 (mg/ml) | |
| 4 | 1 | drop | sodium potassium phosphate | 5 (mM) | pH5.0 |
| 5 | 1 | reservoir | sodium potassium monobasic/dibasic phosphate buffer | 2.4 (M) | pH5.0 |
