1MFW

STRUCTURE OF N-TERMINAL DOUBLECORTIN DOMAIN FROM DCLK: SELENOMETHIONINE LABELED PROTEIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-29
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795,0.9791,0.9718
Spacegroup name	P 1 21 1
Unit cell lengths	38.785, 29.430, 40.099
Unit cell angles	90.00, 115.72, 90.00

Refinement procedure

Resolution	10.000 - 1.600
R-factor	0.13212
Rwork	0.127
R-free	0.18000 *
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.820	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.038	0.210
Total number of observations	28275 *
Number of reflections	9312
<I/σ(I)>	32.5
Completeness [%]	84.6	35.9
Redundancy	3.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	21 *	CITRATE BUFFER, AMMONIUM SULFATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	2.2-2.4 (M)
3	1	reservoir	sodium citrate	0.1 (M)	pH5.2-5.8