1MFA

STRUCTURE OF A SINGLE-CHAIN FV FRAGMENT COMPLEXED WITH A CARBOHYDRATE ANTIGEN AT 1.7 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	53.100, 61.000, 74.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.700
R-factor	0.166
Rwork	0.166
RMSD bond length	0.013
RMSD bond angle	2.800
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Rmerge	0.049 *
Total number of observations	177018 *
Number of reflections	22712 *
Completeness [%]	84.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	19 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	triaccharide	10 (mM)	0.0005 ml
4	1	reservoir	PEG8000	14 (%(w/v))
5	1	reservoir	Tris-HCl	50 (mM)
6	1	reservoir		0.1 (M)