1ME7
Inosine Monophosphate Dehydrogenase (IMPDH) From Tritrichomonas Foetus with RVP and MOA bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 155.068, 155.068, 155.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.150 |
| Rwork | 0.233 |
| R-free | 0.26500 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ak5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.072 | 0.491 |
| Total number of observations | 620781 * | |
| Number of reflections | 35250 * | |
| <I/σ(I)> | 2.05 | 2.05 |
| Completeness [%] | 94.0 | 79.5 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | sodium malonate, Tris, 2-mercaptoethanol, EDTA, glycerol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH8. |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | glycerol | 10 (%) | |
| 5 | 1 | drop | 2-mercaptoethanol | 1 (mM) | |
| 6 | 1 | reservoir | sodium malonate | 42 (%sat) | |
| 7 | 1 | reservoir | Tris | 100 (mM) | pH8.0 |
| 8 | 1 | reservoir | PEG400 | 4 (mM) | |
| 9 | 1 | reservoir | 2-mercaptoethanol | 1 (mM) |
